METHODS & TECHNIQUES | ruizhurtado

Cardiovascular Techniques

Expression and activity of different markers of oxidative damage and antioxidant defense using Absorbance, Fluorescence and Luminescence Techniques

Analysis of intracellular calcium movements in adult cardiomyocytes using Confocal Microscopy

Isolation of adult cardiomyocytes from rat and mouse hearts by digestion with collagenase using Langerdorff Perfusion

Cardiac function and morphology through cardiac imaging analysis using Nuclear Magnetic Resonance

Blood pressure and ECG analysis using Physiological Monitoring System for small animals

Expression and activity of proteins using Western Blot and Zymography

Interaction of proteins using AlphaLISA Tecnology

Localization and co-localization of cellular proteins using Immunofluorescence

Experimental model of Post-Myocardial Infarction in mouse through left anterior descending (LAD) coronary artery ligation

Experimental model of chronic kidney disease in rat or mouse through Nephrectomy 5/6

Urine analysis of different experimental murine models using Metabolic Cages for determining renal function