Cardiorenal Translational
Laboratory
Cardiovascular Techniques
Biochemical Tecniques
Expression and activity of different markers of oxidative damage and antioxidant defense using Absorbance, Fluorescence and Luminescence Techniques
Analysis of intracellular calcium movements in adult cardiomyocytes using Confocal Microscopy
Isolation of adult cardiomyocytes from rat and mouse hearts by digestion with collagenase using Langerdorff Perfusion
Cardiac function and morphology through cardiac imaging analysis using Nuclear Magnetic Resonance
Blood pressure and ECG analysis using Physiological Monitoring System for small animals
Expression and activity of proteins using Western Blot and Zymography
Interaction of proteins using AlphaLISA Tecnology
Localization and co-localization of cellular proteins using Immunofluorescence
Experimental model of Post-Myocardial Infarction in mouse through left anterior descending (LAD) coronary artery ligation
Renal Techniques
Experimental model of chronic kidney disease in rat or mouse through Nephrectomy 5/6
Urine analysis of different experimental murine models using Metabolic Cages for determining renal function